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发布于:2019-10-17 11:25:36  访问:7 次 回复: 篇
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MrsR2 mrsF4 mrsR3 mrsF5 mrsR4 mrsF6 mrsR5 mrsF7 mrsR6 sboA1 sboA
MrsR2 mrsF4 mrsR3 mrsF5 mrsR4 mrsF6 mrsR5 mrsF7 mrsR6 sboA1 sboA2 ablB1 ablBSequence 5‘-3‘ GTGAAGGCCGTACTTTTGTCTGGC GGGGGGAAATACAGCTTCAGGGC GCCCGAAACGGCAGCGCCTG CGGAATGGAGGCTTTGATCCTCTG CGCAAAAGCTCTTCGACCGCCGTC CTCTCGTGCCGTCGGAATATCCGC CGGAGTGAAACCGTGCCGGGATAAAGA GACCATTCAGAGCGGAAAGCTCC GGTGCGGCATGAAGATTTGAGATCACCG GGAGAGCACTTCAATTCCGACGTTGACC GCCTAGATGACATGGCGGCGG GCCGCCGTAGTCGTCCGTGAAGACCG CGCCTAAAGTAGCGCCGCCATCAACGC CCGCGATGGAGCGGGATTATCCG CCTCGCTCCGCATGATCTTTTGG CGGGAGCACGGTGGCAATGTG GTTTCATGGCGGCGAGCACG PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27680749 GATTCGCGGGAAGCGGATTGAGC CTGCCTGCGTATATGATTCCGGC CCGTGATGATGCCGTTCTTCAATCC CGCCCGTGAAGGAGCAGCCG GCCAGGAAGCGGGGCTTCAC CGGCTGCGGGGGAAAAGATCCG CAGCATCAGGGCGTGTATGACCTTC GGAAGAAAAACAGTCGAGGCGATGCTG GAGAAGCTCCGCCGTCACCAGTG GCCCGTGAGCCGAATGGATAAG CCGTTTCAGGGACACAAGCTCCG CCGTTCGCAGGAGGCTATTCC CGCCCATCCTGCTGAAAAAGCG GAAGAGATCCTCGCCAAACATCCGG GAGCGGATTGATCCCGCCGTC GGAGGAGCTAATGACCCATCC CTCCTATGGAGCACGATCCAAC GGAATGCTGGTCCATTCAGCC CAATCGCCAGTATCCTCGCAG CCCAACTTATTTCACCGCCC GTAAGGCTCGGCATTGAATCGAG GGTGTGAAATCGTTGCGTTGG CAACAGGTAGCGTATGCGTGC CGTACAGCCGGGCCAACTTCAAC GGGCGTGCATATTAGGTGGAATC TGAGTGATCGTCAGGCAGCCTTAG TTCTTCATAAGAATACCACGAACCGC CTTTTGCGATCAGAAGGTGCGTATCCG GCCATTATAGAGGAACTGACGATTTC CTTGTGCCAATTCCCGGCTGAC GGATGGCCGGTGTCTCACATG CCATCGGTTTTCCCCATACCCATG GTGGGGGGAGTTTTATGGCGGAG GGTGAAGCCATCAGTGTCCGG CCAGCACCATTCGGTCCAAGAAAACC GTGGCTGTCTCAAACAGAACCGG GTTGCGGCTAATGGAAAACCCAGACC GGGCCTTTGTTAGGTGTATCCCTGG GGAAGACTCCCGCTTATGCCTAAC CCAGTGAACATGAGGAGCCC CGCGATGACAAAAGAAGTCGCCG CTTCATTTGTTCCGCAATGTTCA CCCAGTGGGCCAATTGAATCCTCCC CGCGCAAGTAGTCGATTTCTAACA CAAGTTTGGGCAAAAGAGCTTTTTCMetabolite, gene or flanking area Bacilysin, appropriate Bacilysin, right Bacillaene, left Bacillaene, remaining Bacillaene, right Bacillaene, 616204-22-9 correct Difficidin, still left Difficidin, left Difficidin, suitable Difficidin, ideal Bacillibactin, remaining Bacillibactin, remaining Bacillibactin, right Bacillibactin, appropriate Fengycin, still left Fengycin, remaining Fengycin, proper Fengycin, appropriate Iturin A, still left Iturin A, remaining Iturin A, ideal Iturin A, right Macrolactin, remaining Macrolactin, still left Macrolactin, appropriate Macrolactin, proper Surfactin, left Surfactin, remaining Surfactin, ideal Surfactin, correct Bacilysin, remaining Bacilysin, remaining nrsAB nrsAB nrsC nrsC nrsD nrsD nrsED nrsED nrsF nrsF recA recA recN recN mrsK2 mrsK2 mrsK2R2 mrsK2R2 mrsR2 mrsR2 mrsG mrsG mrsE mrsE mrsE-fbabB mrsE-fbabB sboA sboA albA albAPage 9 of(page PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23157726 range not for citation needs)Microbial Mobile Factories 2009, 8:http://www.microbialcellfactories.com/content/8/1/recN-recA concatenated sequences of associated species in the genus Bacillus were applied applying Muscle software package [52]. A optimum chance phylogenetic tree was constructed via the parsinomy technique applying PAUP* computer software (version 4.0b8, [53]) as well as the sequence from Clostridium botulinum KBAB4 as outgroup. The parameters utilized for tree development were being as follows: (i) model of CS-4718 cost nucleotide substitution: GTR furthermore gamma distribution; (ii) quantity of substitution charge types: six; (iii) charge matrix parameter: approximated by program; (iv) quantity of bloodstrap replicates: one hundred.Genome characterisation For partial genome sequencing, a CS-4326 random shotgun tactic was utilised. Total genomic DNA from strain GA1 was shared by nebulisation according to Surzycki [54]. DNA 875320-29-9 biological activity fragments of 2-3 kb in size were cloned into pMOSblue (GE Health care, Uppsala, Sweden) to determine a shotgun library. The inserts of 500 recombinant plasmids have been sequenced from both of those finishes. Sequences had been processed with Vector NTI software (Invitrogen) and.MrsR2 mrsF4 mrsR3 mrsF5 mrsR4 mrsF6 mrsR5 mrsF7 mrsR6 sboA1 sboA2 ablB1 ablBSequence 5‘-3‘ GTGAAGGCCGTACTTTTGTCTGGC GGGGGGAAATACAGCTTCAGGGC GCCCGAAACGGCAGCGCCTG CGGAATGGAGGCTTTGATCCTCTG CGCAAAAGCTCTTCGACCGCCGTC CTCTCGTGCCGTCGGAATATCCGC CGGAGTGAAACCGTGCCGGGATAAAGA GACCATTCAGAGCGGAAAGCTCC GGTGCGGCATGAAGATTTGAGATCACCG GGAGAGCACTTCAATTCCGACGTTGACC GCCTAGATGACATGGCGGCGG GCCGCCGTAGTCGTCCGTGAAGACCG CGCCTAAAGTAGCGCCGCCATCAACGC CCGCGATGGAGCGGGATTATCCG CCTCGCTCCGCATGATCTTTTGG CGGGAGCACGGTGGCAATGTG GTTTCATGGCGGCGAGCACG PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27680749 GATTCGCGGGAAGCGGATTGAGC CTGCCTGCGTATATGATTCCGGC CCGTGATGATGCCGTTCTTCAATCC CGCCCGTGAAGGAGCAGCCG GCCAGGAAGCGGGGCTTCAC CGGCTGCGGGGGAAAAGATCCG CAGCATCAGGGCGTGTATGACCTTC GGAAGAAAAACAGTCGAGGCGATGCTG GAGAAGCTCCGCCGTCACCAGTG GCCCGTGAGCCGAATGGATAAG CCGTTTCAGGGACACAAGCTCCG CCGTTCGCAGGAGGCTATTCC CGCCCATCCTGCTGAAAAAGCG GAAGAGATCCTCGCCAAACATCCGG GAGCGGATTGATCCCGCCGTC GGAGGAGCTAATGACCCATCC CTCCTATGGAGCACGATCCAAC GGAATGCTGGTCCATTCAGCC CAATCGCCAGTATCCTCGCAG CCCAACTTATTTCACCGCCC GTAAGGCTCGGCATTGAATCGAG GGTGTGAAATCGTTGCGTTGG CAACAGGTAGCGTATGCGTGC CGTACAGCCGGGCCAACTTCAAC GGGCGTGCATATTAGGTGGAATC TGAGTGATCGTCAGGCAGCCTTAG TTCTTCATAAGAATACCACGAACCGC CTTTTGCGATCAGAAGGTGCGTATCCG GCCATTATAGAGGAACTGACGATTTC CTTGTGCCAATTCCCGGCTGAC GGATGGCCGGTGTCTCACATG CCATCGGTTTTCCCCATACCCATG GTGGGGGGAGTTTTATGGCGGAG GGTGAAGCCATCAGTGTCCGG CCAGCACCATTCGGTCCAAGAAAACC GTGGCTGTCTCAAACAGAACCGG GTTGCGGCTAATGGAAAACCCAGACC GGGCCTTTGTTAGGTGTATCCCTGG GGAAGACTCCCGCTTATGCCTAAC CCAGTGAACATGAGGAGCCC CGCGATGACAAAAGAAGTCGCCG CTTCATTTGTTCCGCAATGTTCA CCCAGTGGGCCAATTGAATCCTCCC CGCGCAAGTAGTCGATTTCTAACA CAAGTTTGGGCAAAAGAGCTTTTTCMetabolite, gene or flanking area Bacilysin, right Bacilysin, right Bacillaene, left Bacillaene, remaining Bacillaene, correct Bacillaene, ideal Difficidin, left Difficidin, still left Difficidin, proper Difficidin, correct Bacillibactin, remaining Bacillibactin, left Bacillibactin, ideal Bacillibactin, appropriate Fengycin, still left Fengycin, left Fengycin, appropriate Fengycin, proper Iturin A, left Iturin A, still left Iturin A, suitable Iturin A, proper Macrolactin, still left Macrolactin, left Macrolactin, ideal Macrolactin, proper Surfactin, remaining Surfactin, left Surfactin, proper Surfactin, right Bacilysin, still left Bacilysin, left nrsAB nrsAB nrsC nrsC nrsD nrsD nrsED nrsED nrsF nrsF recA recA recN recN mrsK2 mrsK2 mrsK2R2 mrsK2R2 mrsR2 mrsR2 mrsG mrsG mrsE mrsE mrsE-fbabB mrsE-fbabB sboA sboA albA albAPage 9 of(website page PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23157726 amount not for citation functions)Microbial Mobile Factories 2009, eight:http://www.microbialcellfactories.com/content/8/1/recN-recA concatenated sequences of similar species in the genus Bacillus were being implemented applying Muscle software program [52].
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